Priyanka <br/><br/>
Structural and functional insights into hybrid AT-less megaenzyme synthase (NRPS) and DNA-MsDps2 complexes using single particle cryo-EM 

 - Banglore  IISc  2023 
 - xviii, 177p.  col. ill. ;  29.1 cm * 20.5 cm  e-Thesis  148.6Mb 
<br/><br/> includes bibliographic reference and index 
<br/><br/>PhD; 2023; Molecular biophysics unit 
<br/><br/> Microorganisms, mainly bacteria and fungi, are the producers of structurally diverse, complex organic compounds called as secondary metabolites. These metabolites include polyketides (PKs), non-ribosomal peptides (NRPs), and hybrid PKs/NRPs. The final products from these three classes display different characteristics like antibiotics, antiparasitic agents, antifungals, anticancer drugs, and immunosuppressants. As these products have wide range of potential application in pharmaceuticals, a number of biochemical studies have been carried out to elucidate of their biosynthetic pathways. The biosynthesis of these products is catalysed by large, multi-modular proteins including the NRPSs (non-ribosomal peptide synthases), the PKSs (polyketide synthases) and hybrid NRPS/PKS. Knowledge of the quaternary structure of PKS, NRPS and hybrid NRPS/PKS assembly line enzymes have been topic of interest since it helps not only in elucidating the crosstalk between different domains but also useful in modification of products.  
<br/><br/><label>Subjects--Topical Terms: </label><br/>cryo-EM<br/>MsDps2-DNA<br/>NRPS<br/>polyketide synthases<br/>DNA binding
<br/><br/><label>Dewey Class. No.: </label>570  / PRI