Structural and functional insights into hybrid AT-less megaenzyme synthase (NRPS) and DNA-MsDps2 complexes using single particle cryo-EM
- Bangalore IISc 2023
- xviii, 173p. col. ill. ; 29.1 cm * 20.5 cm e-Thesis 148.1Mb
include bibliographic reference and index
PhD; 2023; Molecular biophysics unit
Microorganisms, mainly bacteria and fungi, are the producers of structurally diverse, complex organic compounds called as secondary metabolites. These metabolites include polyketides (PKs), non-ribosomal peptides (NRPs), and hybrid PKs/NRPs. The final products from these three classes display different characteristics like antibiotics, antiparasitic agents, antifungals, anticancer drugs, and immunosuppressants. As these products have wide range of potential application in pharmaceuticals, a number of biochemical studies have been carried out to elucidate of their biosynthetic pathways. The biosynthesis of these products is catalysed by large, multi-modular proteins including the NRPSs (non-ribosomal peptide synthases), the PKSs (polyketide synthases) and hybrid NRPS/PKS. Knowledge of the quaternary structure of PKS, NRPS and hybrid NRPS/PKS assembly line enzymes have been topic of interest since it helps not only in elucidating the crosstalk between different domains but also useful in modification of products. It has been demonstrated in the literature that FAS and PKS are homodimeric enzyme complexes, whereas the majority of NRPS are monomeric in nature.