Insights on the evaporation and precipitation of pathogen-laden respiratory droplets on inanimate surfaces / (Record no. 433424)
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| 000 -LEADER | |
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| fixed length control field | 05318nam a2200337 4500 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 250413b |||||||| |||| 00| 0 eng d |
| 041 ## - LANGUAGE CODE | |
| Language code of text/sound track or separate title | en |
| 082 ## - DEWEY DECIMAL CLASSIFICATION NUMBER | |
| Classification number | 614.49 |
| Item number | RAS |
| 100 ## - MAIN ENTRY--PERSONAL NAME | |
| Personal name | Rasheed, Abdur |
| 245 ## - TITLE STATEMENT | |
| Title | Insights on the evaporation and precipitation of pathogen-laden respiratory droplets on inanimate surfaces / |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT) | |
| Place of publication, distribution, etc | Bangalore : |
| Name of publisher, distributor, etc | Indian Institute of Science, |
| Date of publication, distribution, etc | 2024. |
| 300 ## - PHYSICAL DESCRIPTION | |
| Extent | xxii, 163 p. : |
| Other physical details | col. ill. |
| Accompanying material | e-Thesis |
| Size of unit | 12.33 Mb |
| 500 ## - GENERAL NOTE | |
| General note | Includes bibliographical references |
| 502 ## - DISSERTATION NOTE | |
| Dissertation note | PhD;2024;Mechanical Engineering |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc | Disease transmission via fluid ejections from infected individuals is a critical public health concern, particularly in high-risk environments like hospitals, public transport, restaurants, and schools. Respiratory droplets containing pathogens such as viruses and bacteria, once deposited on surfaces, can dry and form fomite deposits, facilitating secondary transmission through contact and ingestion. Understanding the evaporation, fluid flow, and precipitation dynamics of such droplets is crucial, yet the interplay between these factors in protein-salt biofluid droplets, and their collective impact on pathogen distribution and survival across varying environmental and surface conditions, remains underexplored. This thesis investigates the evaporation dynamics, internal fluid flow, and pathogen deposition patterns of surrogate respiratory droplets, made of mucin and NaCl, on different substrates (glass, ceramic, steel, PET). Using virus-emulating particles (VEP) and techniques like fluorescence and SEM imaging, the distribution of these particles in dried deposits is studied. Shadowgraphy and optical microscopy track evaporation and precipitation, while MicroPIV reveals internal flow dynamics. Confocal imaging shows pathogen-like particle distribution, highlighting the influence of interfacial transport processes on fomite formation. All droplets exhibited constant contact radius evaporation mode, with internal flows combining capillary and Marangoni effects, resulting in varied edge deposits. Crystallization patterns differed by substrate, and VEP concentration was highest near the three-phase contact line and crystal surfaces, underscoring the role of interfacial processes in fomite-driven infection, especially during COVID-19. Additionally, this study addresses the under-researched topic of droplet behaviour on inclined surfaces. Surrogate respiratory droplets, doped with 0.86μm bacteria-like particles, were studied on substrates inclined at 0°, 45°, and 90°. Results show significant alterations in evaporation, flow, and crystallization dynamics due to inclination, leading to varied nucleation and crystal growth. Confocal microscopy and optical profiling reveal higher bacterial accumulation at the bottom edge, suggesting increased pathogen survival on inclined fomites. To understand the effect of drying on pathogen morphology and the effect of pathogen distribution in deposits on the survival, bacteria suspended in nano pure water is studied. An investigation on flow and desiccation-driven self-assembly of Klebsiella Pneumoniae in the naturally evaporating sessile droplets is conducted. Klebsiella Pneumoniae exhibits extensive changes in its morphology and forms unique patterns as the droplet dries, revealing hitherto unexplored rich physics governing its survival and infection strategies. Klebsiella pneumoniae bacteria also demonstrated unique self-assembly behaviours in evaporating droplets, with a preference for survival in the contact line’s "coffee ring" deposits. Real bacterium Pseudomonas Aeruginosa (PA) was introduced into respiratory droplets to study pathogen survivability under different environmental conditions. A fivefold difference in PA survivability was observed under varying relative humidity (RH) and temperature conditions, illustrating how fomite-mediated disease transmission may vary by geography. For instance, London’s average RH (71%) and temperature (11°C) in 2023 differs significantly from New Delhi's (45% RH, 26°C), suggesting that fomite infection pathways could have demographic dependencies. Lower evaporation rates, particularly under high RH and low temperature, extended crystallization times and reduced internal flows, leading to denser bacterial deposits and enhanced pathogen survivability. Further investigations into the effects of salt and mucin concentrations revealed that elevated mucin increased PA viability tenfold, while salt had minimal impact. Adhesive properties of the deposits were analysed, showing significant pathogen transfer under elevated RH. These insights have critical implications for disease control, influencing sanitization strategies, surface design, and infection prevention efforts. |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name as entry element | Respiratory fluid droplet |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name as entry element | Evaporation |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name as entry element | Pathogen survivability |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name as entry element | Fomites |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name as entry element | Disease transfer |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name as entry element | Biofluid interfaces |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name as entry element | Interfacial phenomena |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name as entry element | Respiratory droplets |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name as entry element | Klebsiella Pneumoniae |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name as entry element | Pseudomonas Aeruginosa |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name as entry element | Droplets |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name as entry element | Bacteria |
| 700 ## - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Advised by Basu, Saptarshi |
| 856 ## - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | https://etd.iisc.ac.in/handle/2005/6862 |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Koha item type | Thesis |
No items available.
