Determinants of protein stability and function inferred from saturation mutagenesis and next-generation sequencing (Record no. 432314)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 01764nam a22002177a 4500 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 240630b |||||||| |||| 00| 0 eng d |
| 082 ## - DEWEY DECIMAL CLASSIFICATION NUMBER | |
| Classification number | 572.6 |
| Item number | BHA |
| 100 ## - MAIN ENTRY--PERSONAL NAME | |
| Personal name | Bhasin, Munmun. |
| 245 ## - TITLE STATEMENT | |
| Title | Determinants of protein stability and function inferred from saturation mutagenesis and next-generation sequencing |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT) | |
| Place of publication, distribution, etc | Bangalore : |
| Name of publisher, distributor, etc | Indian Institute of Science, |
| Date of publication, distribution, etc | 2024. |
| 300 ## - PHYSICAL DESCRIPTION | |
| Extent | xiv, 413 p. : |
| Other physical details | col. ill. |
| Accompanying material | e- Thesis. |
| Size of unit | 14.23 Mb. |
| 500 ## - GENERAL NOTE | |
| General note | Includes reference. |
| 502 ## - DISSERTATION NOTE | |
| Dissertation note | PhD;2024;Molecular Biophysics Unit. |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc | Proteins play a central role in all biological processes. Despite a similar underlying composition, they exhibit immense diversity in structures and functions, a subject of great interest. The research presented in this thesis aims to deepen our understanding of the relationships between protein structure and function by describing various techniques developed to investigate these relationships. Deep sequencing of synonymous mutations in the E. coli CcdB toxin revealed complex interactions affecting protein expression and function, highlighting the nuanced roles of translational control and codon specificity. RNA sequencing provided insights into the differential gene expression and RNA cleavage specificities of various toxins in M. tuberculosis, enhancing our understanding of the physiological roles of these toxin-antitoxin systems. Our studies on the MazEF6, MazEF9, VapBC13, and VapBC26 systems have provided valuable insights into their regulatory roles and RNA cleavage patterns. |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name as entry element | RNA sequencing |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name as entry element | Protein stabilization |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name as entry element | Deep mutational scanning |
| 700 ## - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Advised by Varadarajan, Raghavan. |
| 856 ## - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | https://etd.iisc.ac.in/handle/2005/6539 |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Koha item type | Thesis |
No items available.
