Studies on bodipy-platinum(Ii/Iv) conjugates for cellular imaging and photodynamic therapy (Record no. 431102)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 03787nam a22002297a 4500 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 231206b |||||||| |||| 00| 0 eng d |
| 082 ## - DEWEY DECIMAL CLASSIFICATION NUMBER | |
| Classification number | 546 |
| Item number | BER |
| 100 ## - MAIN ENTRY--PERSONAL NAME | |
| Personal name | Bera, Arpan |
| 245 ## - TITLE STATEMENT | |
| Title | Studies on bodipy-platinum(Ii/Iv) conjugates for cellular imaging and photodynamic therapy |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT) | |
| Place of publication, distribution, etc | Bangalore: |
| Name of publisher, distributor, etc | Indian Institute of Science, |
| Date of publication, distribution, etc | 2023. |
| 300 ## - PHYSICAL DESCRIPTION | |
| Extent | xviii, 377p.: |
| Other physical details | col. ill. |
| Accompanying material | e-Thesis |
| Size of unit | 25.86 MB |
| 500 ## - GENERAL NOTE | |
| General note | Includes bibliographical references and index |
| 502 ## - DISSERTATION NOTE | |
| Dissertation note | PhD; 2023; Inorganic and Physical Chemistry |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc | Cancer emerges from the abnormal proliferation of aberrant cells. Amidst a multitude of treatment options, chemotherapy is widely used as a viable methodology. Despite its widespread use, it is encumbered with significant limitations due to drug related side-effects. Consequently, in recent times, photodynamic therapy (PDT) and photoactivated chemotherapy (PACT) have emerged as highly promising alternatives to traditional chemotherapy. This thesis aims to conceive and synthesize new platinum(II/IV) complexes using ligands as multifunctional agents for effective phototherapy and photochemotherapy applications. In the initial phase, a series of mono-functional platinum(II) complexes [Pt(L1-3)Cl]Cl (1-3) were synthesized having BODIPY (boron-dipyrromethene)-tagged dipicolylamine (dpa) ligands (L2 and L3) as green and red light emitting photosensitizers along with a benzyl derivative of dpa (L1). These mono-functional complexes exhibit remarkable affinity for DNA binding, as evidenced from a study employing the model nucleobase 9-EtG, while also displaying the ability to generate singlet oxygen upon photoactivation of the BODIPY photosensitizer. In subsequent investigations, we synthesized a novel platinum(IV)-BODIPY conjugate, named Oxoplatin-B (4), derived from cisplatin. The prodrug exhibited photo-induced ligand release, accompanied by the simultaneous formation of the cisplatin core through a two-electron Pt(IV)-Pt(II) reduction process. Encouraged from the results obtained with the green light Pt(IV) prodrug, we embarked on investigating the possibilities of a clinically applicable platinum(IV) prodrug with the formulation [Pt(NH3)2Cl2(OH)(L6)] (6), having a red light active BODIPY ligand (HL6). Upon red light irradiation, the complex exhibited significant PDT activity with sub-micromolar IC50 values in cancer cells such as HeLa and MCF-7. Moving forward, we introduced a novel prodrug (7) formulation that combines a platinum(IV) core with a bioactive biotin (vitamin B7) moiety, covalently linked to a PEGylated BODIPY ligand (HL7) designed to respond to red light stimulation and for enhanced bioavailability. Notably, the complex displays remarkable stability in solution in dark, but rapidly activates upon red light exposure without any need for external reducing agents. Finally, we made a comprehensive study on the synthesis and characterization of a hetero-bimetallic Ru(II)-Pt(IV) conjugate (9). This conjugate presents a dual-action Pt(IV) complex with a chemo-active unit and a BODIPY-Ru(II)-bis-terpyridine dyad complex as a photosensitizer. This prodrug induced significant photocytotoxicity with remarkably high phototherapeutic index (PI) value of >4500 in A549 cancer cells. The findings of this thesis work pave the way for innovative approaches in the design and development of new generation of platinum(II/IV) complexes having BODIPY-based photosensitizers, offering exciting prospects in PDT and cellular imaging applications. |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name as entry element | Platinum complexes |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name as entry element | Boron dipyrromethene |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name as entry element | Crystal structure |
| 700 ## - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Advised by Das, Debasis |
| 700 ## - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Advised by Chakravarty, Akhil R |
| 856 ## - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | https://etd.iisc.ac.in/handle/2005/6313 |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Koha item type | Thesis |
No items available.
